RAPD analysis of genetic differentiation in the different hosts and geographic populations of Drosicha corpulenta

The genetic diversity and population differentiation among geographic populations of 16 different Drosicha corpulenta host collected from Baoding,Shijiazhuang,and Handan in Hebei were analyzed by RAPD. The results showed that four RAPD primers were amplified out of 41 polymorphism loci,and that the percent of polymorphic loci was 100%.The genetic distance index of the populations ranged from 0. 0701 to 0. 4360 and the average value was 0. 2395. The genetic distance index for D. corpulenta populations on Pterocarya stenoptera and Malus halliana( Voss.) Koehne in Handan was the smallest( 0. 0701). The genetic distance index for D. corpulenta populations on Prunus cerasifera in Shijiazhuang and Hibiscus syriacus in Handan was the largest of all,and the similarity coefficients ranged from 0. 6466 to 0. 9290. The genetic diversity of the different D. corpulenta populations was classified as abundant,and genetic differences were found.According to the results of cluster analysis,the genetic diversity of D. corpulenta populations was affected by both host and geographic location,and the different host populations of D. corpulenta showed obvious genetic differentiation.