S-S Synapsis during Class Switch Recombination Is Promoted by Distantly Located Transcriptional Elements and Activation-Induced Deaminase
2007
Summary Molecular mechanisms underlying synapsis of activation-induced deaminase (AID)-targeted S regions during class switch recombination (CSR) are poorly understood. By using chromosome conformation capture techniques, we found that in B cells, the Eμ and 3′Eα enhancers were in close spatial proximity, forming a unique chromosomal loop configuration. B cell activation led to recruitment of the germline transcript (GLT) promoters to the Eμ:3′Eα complex in a cytokine-dependent fashion. This structure facilitated S-S synapsis because Sμ was proximal to Eμ and a downstream S region was corecruited with the targeted GLT promoter to Eμ:3′Eα. We propose that GLT promoter association with the Eμ:3′Eα complex creates an architectural scaffolding that promotes S-S synapsis during CSR and that these interactions are stabilized by AID. Thus, the S-S synaptosome is formed as a result of the self-organizing transcription system that regulates GLT expression and may serve to guard against spurious chromosomal translocations.
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