Construction and ETS analysis on full-length cDNA plasmid library of Taenia saginata adult worms.

The objective of the present study was to construct the full-length cDNA plasmid library for adult worms of Taenia saginata and then to form a gene expression pattern in order to establish a foundation for effective vaccine candidate antigen research and for comparative study of three main kinds of human Taenia. The mRNA was extracted from the T. saginata adult worms and the cDNA was synthesized. Products were digested by proteinase K and SfiⅠ, and then fractionated by CHROMA SPIN -400 column. The fractions were characterized by 1.1% agarose/EB gel electrophoresis, and the full-length cDNA plasmid library pBluescript II SK was constructed. The titer of the amplified library was evaluated and the quality of the constructed library was tested by PCR amplification using amphi-primers of the vector cloning sites.Furthermore,the positive recombinant clones were carried out with the 5’ terminus sequencing in large scale and then merged to unigenes. The results indicated that the titer of amplified library was 1016 pfu/μL. Of 1023 clone sequences were obtained and in with 419cDNA were merged. It was evident that the high-quality full-length cDNA plasmid library of adult T. saginata has been constructed successfully.