Low-temperature stress strengthening pulsed electric field method of killing bacteria Listeria monocytogenes

The present invention discloses a method of low-temperature stress Listeria monocytogenes enhanced killing pulsed electric field, comprising the steps of: (1) Fresh liquid sample; (2) a liquid sample is first stored program Cooling: 13 ~ storage at 17 ℃ 1 ~ 2h, the temperature was then lowered to 3 ~ 7 ℃ storage 1 ~ 2h; (3) the liquid sample was warmed to 35 ~ 40 ℃, pulsed electric field treatment immediately. The present invention can achieve an effective kill of L. monocytogenes results in a lower electric field strength pulse, more efficient, safe, energy-saving.