Expression of prostate apoptosis response-4 in PC12 cells after retonone treatment.

Objective To study the prostate apoptosis response-4(Par-4) expression in PC12 cells after retonone treatment and to provide a new target for the treatment of Parkinson′s disease(PD).Methods: 1,5,or 10 μmol/L rotenone was given to PC12 cells for 6 h and 24 h.MTT assay was used for determining the viability rate,and western blot was used to determine the protein level of Par-4.Results After rotenone treatment,cells showed different toxic appearances.After the 6h-treatment,the photo-density ratio of Par-4 to actin in the 10 μmol/L group(P=0.029 646)was significantly increased compared with the normal control group.After being pre-treated with 20 μmol/L Ginestin,an inhibitor of the JNK pathway,for 1 hour,the ratio was decreased.However in the 24 h groups,the viability rates significantly decreased,and ratios in the 1, 5 μmol/L(P=0.018 461,P=0.043 415) groups were increased compared with the 10 μmol/L group(P=0.081 298).Conclusion Expression of Par-4 in PC12 cells is increased after rotenone treatment in a time-and concentration-dependent manner.The JNK pathway participates in the process of Par-4 stepping up.