Harmonease Chewable Tablets reduces noise-induced fear and anxiety in a laboratory canine thunderstorm simulation: A blinded and placebo-controlled study

Thunderstorm simulation in the laboratory setting induces fearful and anxious behavior in beagles, most notably manifested by increased inactivity (''freezing''), which, in a companion study, was ameliorated by the anxiolytic diazepam (Araujo et al., 2009). Using this protocol, the present study assessed the efficacy of Harmonease, a chewable oral anxiolytic botanical product containing a proprietary blend of extracts of Magnolia officinalis and Phellodendron amurense .Ab alanced, placebo-controlled, blinded, single crossover design including 20 healthy adult beagles was used for this study. After a baseline thunderstorm test, subjects received Harmonease Chewable Tablets or pla- cebo treatment daily and were reassessed on the treatment day 7. After a 7-day washout period, the treatments were crossed over and a design identical to that used in the first phase was used. The thunderstorm test was performed in an open-field arena (8 ft ! 9 ft) and consisted of three 3-minute phases: an anticipatory phase in which no stimulus was provided; the thunderstorm phase in which a thunderstorm track was played over a speaker system; and a recovery phase in which no stimulus was presented. Inactivity duration was considered the primary variable for assessing efficacy which was measured by a trained observer. Difference in number of dogs improved versus worsened by treatment group was significant at P , 0.05. Specifically, 12 of 20 (60%) dogs improved from baseline when treated with Harmonease, whereas only 5 of 20 (25%) improved on placebo. Furthermore, 9 of 20 (45%) placebo dogs showed increased inactivity duration (worsened), whereas only 4 of 20 (20%) treatment dogs worsened. Increases in distance travelled consistent with reduced inactivity were also seen under Harmonease. Harmonease reduced fear-related inactivity or freezing in dogs in this thun- derstorm simulation model. This supports past studies demonstrating that the combination of botanical extracts in Harmonease is effective in dogs for the management of stress-related behaviors.