Isolation and sequence analysis of the Schistosoma japonisum cDNA encoding the small subunit of RNA polymerase II transcription facter SIII-p15

Aim To isolate and identify novel genes of Schistosoma japonicum(Sj) from cDNA library Methods cDNA clones were randomly screened from Sj cDNA library by EST stratagem The inserts of positive cDNA clones were identified by PCR direced sequencing and homologous searching of ESTs,the cDNA sequences encoding Sj small subunit of RNA polymerase Ⅱ transcription factor SⅢ was analyzed by sub cloning and bioinformatics Results a cDNA clong was isolated from Sj cDNA library,which sequence included a ORF of 371 bp,and translated into 118 amino acid residues The deduced protein was similar to the corresponding sequence of other biologies Conclusion the full length cDNA sequences encoding Sj small subunit of RNA polymerase Ⅱ transcription facter SⅢ was obtained,which gave the basis for further functional study of this gene