Antimicrobial, antioxidant, and anticancer potentials of AgCl nanoparticles biosynthesized by Flavobacterium panacis

Silver chloride nanoparticles (AgClNps) were biosynthesized extracellularly by Flavobacterium panacis (DCY106T), which was isolated from the rhizosphere of Panax ginseng in Gochang, Republic of Korea. The reduction of silver nitrate by DCY106T was initiated within 24 h at 37 °C. The complete reaction did not require supplemental reducing or stabilizing agents, further demonstrating the facile and simple synthesis of this methodology. The formation of nanoparticles was confirmed by UV–Vis spectroscopy, showing the maximum absorbance at ~ 402 nm. Field emission transmission electron microscopy revealed a spherical structure of the nanoparticles. Furthermore, the average size of AgClNps was around 20.66 nm as determined by X-ray diffraction. Fourier transform infrared spectroscopy analysis showed that functional biomolecules, such as proteins or enzymes from the cell-free supernatant of DCY106T, may provide nanoparticle stabilization against aggregations. In addition, the biosynthesized AgClNps served as competent antimicrobial agents against several pathogenic bacteria and fungi. Antioxidant results determined by 2, 2-diphenyl-1-picrylhydrzyl (DPPH) scavenging assay exhibited an efficient antioxidant activity of AgClNps. Moreover, AgClNps also revealed the anticancer potential against human lung cancer cells. Thus, this study proposes a facile extracellular synthesis of AgClNps by F. panacis (DCY106T) are applicable as the potential antimicrobial, antioxidant, and anticancer agents.