The Effect of Plant Growth Promoting Rhizobacteria on Growth Parameters, Antioxidant Enzymes and Microelements of Canola under Salt Stress

Plant Growth Promoting Rhizobacteria (PGPR) include bacteria (Rhizosphere and Rhizoplane) residing in roots that useful for plants in some conditions. Azospirillum and pseudomonas are some of these bacteria. The bacteria are responsible for physiological and morphological changes in plant. This study was carried out to investigate the effects of PGPR including three species of Azospirillum, A.lipoferum , A.brasilense, Azosprillum lipoferum sp. and two strains of Pseudomonas, P. putida, Pseudomonas fluorescens on growth parameters, Antioxidant enzymes and Microelements. The research was on two Canola cultivars; Hyola 401 and RGS 003 under two salinity levels, 80 and 160 mM NaCl based on randomize complete design as factorial with three replication under green house condition in Leonard medium with Hoagland solution in Department of Water and Soil in Golestan Agricultural and Natural Resources Research Center. The result showed that salinity stress had a significant effect on growth traits. Canola seeds inoculation with PGPR has recovered salinity effects on some growth parameters, and the most effects was related to Azospirillium strains specially A.brasilense. ANOVA results showed that the Maximum Fe and Zn were related to A.lipoferum and Maximum Mn was related to A.lipoferum Sp. Also, Antioxidant enzymes were increased under both salinity levels compare to control, and the increase was more under 160 mM NaCl, so that Canola inoculation with five strains of PGPR caused to decrease the level of enzymes, that A.lipoferum Sp and A.lipoferum had the most effects.