N-(3-Sulfo, 3-carboxy)-propionylchitosan as New Chiral Selector for Enantioresolution of Basic Drugs by Capillary Electrophoresis

N-(3-sulfo, 3-carboxy)-propionylchitosan (SCPC) has been investigated as new chiral selector for capillary electrophoresis. The running buffer species, pH and the chiral selector concentration were the parameters studied to achieve the enantioseparation. The best results were obtained using 2% chiral selector in acetate running buffer. Baseline enantioseparation was obtained for pindolol, fluoxetine and chlorcyclizine. The enantioselectivity of the N-(3-sulfo, 3-carboxy)-propionylchitosans obtained from low-molecular mass (MWη 7 kDa) and high-molecular mass (MWη 200 kDa) chitosans is similar. The N-(3-sulfo, 3-carboxy)-propionylchitosan appear to have a mechanism of enantiorecognition somewhat different from that on others charged polysaccharides due to the presence of an additional chiral center in the monosaccharide residue.