살구 중 Flonicamid 및 그 대사산물의 잔류특성 연구

This study was conducted to investigate residual characteristics of Flonicamid and metabolites among minor crop apricots, evaluate safety and use them as data for expanding the registration of pesticides. The test pesticide was harvested after diluting Flonicamid 10 % water dispersible granule (WG) 2,000 fold and spraying it from 50 days before harvest to 7 days before harvest. 10mL of acetonitrile containing 5% acetic acid was added to 10g ± 0.05g of homogenized sample, and after shaking for 10 minutes, a sample was extracted by adding a QuEChERS (Original)kit. The extracted sample was analyzed for Flonicamid, metabolites TFNA, and TFNG using LC-MS/MS. Standard calibration curves were made by matrix matched standards and their correlation coefficients were higher than 0.99. The method limit of quantitation (MLOQ) was each 0.02 mg/kg for Flonicamid and metabolite TFNA, TFNG. Average recovery rates of Flonicamid, metabolites TFNA, and TFNG were 89.2-110.2 %, 91.4-103.8 %, and 95.8-113.6 %, respectively. The converion residue amounts of Flonicamid at 50-40 DAT (days after treatment) were 0.12- 0.15 mg/kg, 40-30 DAT were 0.16-0.17 mg/kg, 30-21 DAT were 0.10-0,11 mg/kg, 21-14 DAT were 0.11-0.15 mg/kg, 14-7 DAT were 0.11-0.21 mg/kg, respectively. Residual concentration of Flonicamid in the crop was increased as spraying more closer to harvest day. The percentage of estimate daily intake(%EDI) compared to ADI based on the residual amount of Flonicamid in apricots from 0.0018% to 0.0034%. The %ADI was considered safe at less than 10%.