急性肺血栓栓塞大鼠血浆MMP-2和MMP-9的活性变化及意义

Objective To explore the change and significance of plasma MMP-2 and MMP-9 activity in acute pulmonary thromboembolism (PTE).Methods 72 male Sprague-Dawley rats were randomly divided into three groups:Sham group,PTE group and Statins group.Right ventricular systolic pressure and mean pulmonary arterial pressure were measured by right heart catheter at different time points following the induction of PTE.The enzymic activity of MMP-2 and MMP-9 in plasma were detected through gelatin zymography.Results Compared with Sham Group,the plasma MMP-9 activity at every time point in PTE Group increased significantly ( P ＜ 0.05).Compared with PTE Group,the plasma MMP-9 activity at every time point in Statins Group decreased significantly ( P ＜0.05),but was still higher than that of Sham Group ( P ＜0.05).No significant difference in MMP-2 activity at every time point was observed among three groups ( P ＞0.05).Conclusions The plasma MMP-9 activity was increased in the PTE rats,pretreatment with simavastatin attenuated acute PTE-induced pulmonary.hypertension through attenuationing of MMP-9 activity.