Влияние газотрансмиттера сероводорода на микрореологические свойства эритроцитов здоровых лиц и больных сахарным диабетом второго типа

Aim: to study the effect of donor gasotransmitter (GT) hydrogen sulphide (NaHS) and the activator of its intracellular synthesis (L-cysteine) on the red blood cells (RBCs) microrheological characteristics in normal and diabetes mellitus type 2 (DM-2). Materials and methods. RBCs of 16 healthy individuals and 16 patients with DM-2 were incubated with hydrogen sulfide donor — sodium hydrosulfide (NaHS) in concentrations of 10, 50 and 100 μmol and a stimulator of its endogenous synthesis — L-cyste- ine (10, 50 and 100 μmol). ATP-dependent potassium channels were blocked by glibenclamide (50 μmol). Soluble guanylate cy- clase was inhibited using 0.5 μmol ODQ (1H-[1,2,4]-oxadiazolo[4,3-a] quinoxalin-l-one). Deformability (RBCD) and RBCs aggrega- tion were recorded. Results. Under the influence of NaHS, dose-dependent positive changes in RBCs microrheology were observed. Cellular microrhe- ological responses to GT donor were more pronounced in healthy individuals than in patients with DM-2. A similar pattern was observed when RBCs were exposed to L-cysteine. Preincubation with glibenclamide did not eliminate the positive effect of GT donor on RBCs microrheology. Whereas inhibition of soluble guanylate cyclase with the help of ODQ completely eliminated posi- tive changes in RBCD under the action of GT and significantly reduced its effect on aggregation. Conclusion. Thus, hydrogen sulfide as a gasotransmitter improves RBCs microrheology. With the same concentration of hydrogen sulphide donor (NaHS) and its synthesis stimulator (L-cysteine), more pronounced shifts in the microrheological parameters of erythrocytes are observed in healthy individuals as compared with DM-2 patients. REFERENCES 1. Bor-Kucukatay M., Wenby R.B., Meiselman H.J., Baskurt O.K. Ef- fects of nitric oxide on red blood cell deformability. Am J Physiol Heart Circ Physiol. 2003;284(5):H1577–84. DOI: 10.1152/ajp- heart.00665.2002. 2. Popel A.S., Johnson P.C. Microcirculation and hemorheology. 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