Expresion, purificación parcial y estudios computacionales de la IDShr producida en Pichia Pastoris

This work was carried out in order to improve the expression conditions and purification of the hrIDS in and E. coli JM109/pUC13-IDS-28. The work comprises two parts an experimental and a computational one. In relation to the experimental part, a fluorometric test using MU-αIdoA-2S as substrate was used for the detection of the hrIDS, this test was used in order to check that the hrIDS expressed in those biological models was biologically active. Using as antigen the purified hrIDS (donated by TKT), a polyclonal antibody against α-hrIDS was obtained in rabbit, this was used to make an affinity chromatography column, that was employed in the purification trials.