Development and Validation of RP-HPLC method for the determination of Ozagrel HCl

Rapid and sensitive high-performance liquid chromatographic method for determination of ozagrel HCl. in bulk using an internal standard, caffiene was developed. chromatographic separation was accomplished by using a C18 analytical column with a mobile phase consisting of Methanol and 0.1% v/v aqueous formic acid (86: 14 v/v). Ozagrel HCl and the internal standard were detected by ultraviolet absorbance at 276 nm. The lower limits of detection and quantification were both 2 and 10 ng/ml, respectively, and the calibration curves were linear over a concentration range of 0.1–1.0 μg/ml of ozagrel HCl.The method provides a sensitive, accurate and reliable analytical procedure suitable for analysis of Ozagrel HCl in bulk.