116 Prevotella histicola drives transcription of an elevated host response compared to P. nigrescens in cystic fibrosis bronchial epithelial cells

Objectives: P. histicola and P. nigrescens have been isolated from the CF lung, but their contribution to disease pathogenesis is unclear. We hypothesised that these isolates are capable of contributing to the inflammatory response in CF by utilising the Toll like receptor pathway (TLR) to drive transcription of inflammatory cytokines. Methods: CF bronchial epithelial cells (CFBE41o−) were exposed to Prevotella spp. in an anaerobic environment for 4 hours. RNA was extracted from the cells for qPCR for a range of genes associated with innate immunity (Table 1) and nuclear protein was extracted from the cells to assess NF-uB activation (Table 2). Bacterial cellular adherence was simultaneously assessed. Results: See the tables.