Micro RNA-19a interferes with IL-10 expression in peripheral dendritic cells of patients with nasal polyposis

// Xiang-Qian Luo 1, 2, * , Jian-Bo Shao 1, 2, * , Rui-Di Xie 2 , Lu Zeng 2 , Xiao-Xi Li 2 , Shu-Qi Qiu 3 , Xiao-Rui Geng 3 , Li-Tao Yang 3, 4 , Lin-Jing Li 4 , Da-Bo Liu 1 , Zhi-Gang Liu 2 and Ping-Chang Yang 2 1 Department of Otolaryngology, Guangzhou Women and Children’s Medical Center, Guangzhou Medical University, Guangzhou 510010, China 2 The Research Center of Allergy and Immunology, Shenzhen University School of Medicine, Shenzhen 518060, China 3 Longgang ENT Hospital, Shenzhen 518116, China 4 Brain Body Institute, McMaster University, Hamilton, ON, L8N 4A6, Canada * These authors equally contributed to this work Correspondence to: Ping-Chang Yang, email: pcy2356@szu.edu.cn Zhi-Gang Liu, email: lzg@szu.edu.cn Keywords: nose, nasal polyp, dendritic cells, micro RNA, interleukin-10 Received: November 02, 2016      Accepted: March 14, 2017      Published: March 24, 2017 ABSTRACT The pathogenesis of nasal polyp is to be further investigated. Micro RNA (miR) plays a role in the development of allergic inflammation. Interleukin (IL)-10-producing dendritic cells (DC) have immune tolerogenic properties. This study test a hypothesis that miR-17-92 cluster is associated with suppressing IL-10 in peripheral DC. In this study, peripheral blood samples were obtained from 26 patients with nasal polyp. The CD11c DCs were isolated from the blood samples and analyzed for the expression of IL-10. We observed that, as compared with healthy subjects, the IL-10 expression in peripheral DC was significantly lower in polyp patients. The levels of miR-19a, but not the rest 5 members of the miR-17-92 cluster, were markedly higher in DCs in polyp group. Exposure to recombinant IL-4 suppressed the IL-10 expression in DCs, which was abolished by blocking histone deacetylase-11 or knocking down the miR-19a gene in DCs. We conclude that miR-19a plays a critical role in the suppression of IL-10 in peripheral DCs, which may be a target in the immune therapy for nasal polyp.