Intermediate filaments in motion: observations of intermediate filaments in cells using green fluorescent protein-vimentin.

In the movies presented in this essay we have demonstrated that IFs are dynamic in nature. Movements of IFs include both extension and retraction and are dependent on the presence of intact MTs. Furthermore, we have characterized a possible intermediate of IF turnover, IF fragments. These fragments are also dynamic and can be visualized moving at the leading edge of cells migrating into a wound edge. Interestingly, the movement of these fragments is also dependent on intact MTs, suggesting that MTs may play a role in turnover of IFs.