Potential of Xenorhabdus Spp., Photorhabdus Spp. and its Metabolites against Spodoptera Litura F.

In Pakistan very little work has been done on bacteria associated with entomopathogenic nematodes and their metabolites. Keeping in view the mentioned objective following research has been planned. Two bacterial isolates Xenorhabdus spp. associated with Steinernema glaseri and Photorhabdus spp. associated with Heterorhabditis bacteriophora were evaluated against Spodoptera litura (F.) in lab conditions. These isolates were sprayed at the rate of 4×10 7 against different larval stages of Spodoptera litura (F.). After 1 st day maximum mortality caused by Xenorhabdus spp. in 2 nd larval instar which was 59.40% followed by 5 th , 3 rd and 4 th larval instar which was 52.80%, 46.20% and 46.20%. Mortality was found to be 100% in all S. litura (F.) larval instars when treated with bacterial suspension upto 4 days. Maximum mortality was observed in Xenorhabdus spp. against 2 nd larval instar which was 59.40% as compared to Photorhabdus spp. that was 26.40% after 1 st day. Mortality was increased as time increased which was 100%. Similarly bacterial metabolites (40%) were evaluated against different larval stages of S. litura (F.). Metabolites were significantly effective against all larval instars. Mortality was found to be 100% in all S. litura (F.) larval instars when treated with metabolites upto 4 days. Maximum mortality was observed in metabolites which were isolated from Xenorhabdus spp. against 2 nd larval instar which was 52.80% as compared to Photorhabdus spp. which was 26.40% after 1 st day. Mortality increased as time increased which was 100%. Present investigations proved that EPNs has an option for possible management which has enough efficacies against Spodoptera litura .