Preparation and content determination of metadoxine suppository
2014
Objective:To investigate the preparation process and to determine the content of metadoxine suppository.Methods:Suppositories were prepared with metadoxine as the main component.The content of metadoxine in the suppository was determined by HPLC method.The HPLC method was applied on a Gemini 5U-C18column(250mm×4.6mm,5μm),at35℃column temperature,with the mobile phase consisting of acetonitrile-0.1%phosphoric acid solution(1∶99),flow rate of1ml/min and the detection wavelength of 205 nm.Results:The prepared metadoxine suppository was an ecru conical solid.Metadoxine presented good linearity in the range of 20.2-101μg/ml.The standard curve equation was A=56.83 c+47.88(r=0.999 9).The recovery rates of low,medium and high concentrations(40.4,60.6,80.8μg/ml)of metadoxine were(99.97±0.032)%,(99.91±0.076)% and(99.86±0.046)%(n=3),respectively.Conclusion:The method established by this research for suppository preparation is feasible,and the content determination method could be used for the study of metadoxine suppository.
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