Association Analysis of Nitrogen Use Efficiency (NUE) In Brassica campestris L.

In order to illustrate the genetic mechanism of nitrogen use efficiency(NUE)and identify the gene-linked markers for NUE in Brassica campestris L.,we genotyped 160 representative B.campestris accessions using 207 Insertion/Deletion(InDel)locus evenly distributed along the genome.Population structure was firstly analyzed for the population,then the structure-based association analysis between InDel locus and 10 leaf morphology and biomass traits in two contrasting N environments was performed by using TASSEL GLM(general linear model)program.Genetic structure analysis showed that 160 lines were composed of five subpopulations.We identified 45 and 35 markers significantly associated with 10 traits in experiments performed in Yunnan and Beijing,respectively.Among those,4 markers were co-localized in two experiments and the loci identified explained 0.06 of the phenotypic variance on average.There were 86 significant locus were identified only under one N condition,which might due to that N supply condition changed expression or regulation some of genes.