Alveolar bone resorption induced by CD4+CD45RB high-density T-cell transfer in immunocompromised mice.

Background: The aims of this study are to determine whether the antigen-inexperienced (naive, CD45RB high-density) T-cell (CD4+CD45RBHigh T-cell) transfer model is associated with alveolar bone resorption, to elucidate the local osteogenic/adipogenic potential of alveolar bone marrow stromal cells (ABCs) from T-cell–transferred animals, and to investigate the systemic osteogenic potential by transplanting human periodontal ligament stem cells (hPDLSCs) into these animals.Methods: CD4+CD45RBHigh and CD4+CD45RBLow (antigen-experienced [memory, CD45RB low-density]) T cells were sorted and transferred into severe combined immunodeficiency (SCID) mice to induce inflammatory bowel disease–like syndrome (n = 8). hPDLSCs were transplanted into T-cell–transferred SCID mice to examine ectopic cementum formation 8 weeks after T-cell transfer. The mandibles and tibias of these mice were retrieved for microcomputed tomography (micro-CT), histomorphometric analysis, and isolation of ABCs 16 weeks after T-cell transfer....