Isolation and Identification of a New Glycoside in Tobacco and Analysis of its Pyrolytic Products

A new glycoside,(E)-N-[(4-hydroxy-3-methoxy)-phenethyl]-3-[(3-methoxy-4-O-β-D-glucopyranosyloxy) phenyl]acrylamide(Ⅰ),was isolated from leaves of flue-cured tobacco cv."Hongda"through extraction with 95% ethanol,preparation at normal and high pressure and separation with chromatography.The molecular structure of compound(Ⅰ)was identified with HRESI-MS and NMR.Compound(Ⅰ)was pyrolyzed in He atmosphere and its pyrolytic products were analyzed by GC/MS.The results showed that the separated product was compound(Ⅰ),and its major pyrolytic products were 4-vinylguaiacol,guaiacol,vanillin,p-cresol,2,3-dihydrobenzofuran,4-allylphenol,and m-ethylphenol.