Synthesis of 14C‐uridine‐5′ ‐triphosphate by enzymes of escherichia coli cell‐free extracts

Crude cell-free extracts of Escherichia coli AB 259 Hfr 3000, thi-perform multistep conversion of 14C-uracil to 14C-UTP in vitro in the presence of D-ribose/or ribose-5-phosphate/, ATP and ATP-regenerating system. ATP concentration is crucial for significant 14C-UTP production. Quantitative yield of the product combined with relatively simple procedure and inexpensiveness of initial materials makes the method advantageous for large-scale preparation of 14C-UTP.