Establishing method and application of fingerprint of detoxifying and kidney harmonizing capsule

The invention provides an establishing method of the fingerprint of a detoxifying and kidney harmonizing capsule, and an application of the fingerprint. The establishing method comprises the following steps: 1, dissolving the content of the detoxifying and kidney harmonizing capsule in an 70%(V) aqueous ethanol solution to prepare a sample solution having a concentration of 5mg/ml; and 2, detecting the sample solution by adopting a high performance liquid chromatograph-tandem mass spectrometry to obtain a total ionic chromatogram, wherein a high performance liquid chromatograph adopts a C18 reversed-phase bonded silica gel chromatography column, a mobile phase 1 and a mobile phase 2 are used for gradient elution, an electro-spray detector is used for detection, the mobile phase 1 is a 0.1%(V) aqueous solution of formic acid, and the mobile phase 2 is a 0.1%(V) formic acid-acetonitrile solution. The method has the advantages of high detection accuracy and reliability when the method is used for obtaining the fingerprint, and the obtained fingerprint can rapidly, simply, comprehensively and effectively display the effective components of the detoxifying and kidney harmonizing capsule and the contents of the effective components.