Detection of Citrus tatter leaf virus (CTLV) by real-time RT-PCR.

Citrus tatter leaf disease caused by citrus tatter leaf virus(CTLV) is an economically important systemic disease of citrus in the world.So it is essential to develop a rapid and reliable method to detect CTLV.Using a pair of primers ASG-Pf and ASG-Pr,a reatime RT-PCR system based on SYBR Green I dye has been established to detect CTLV.The specificity, sensitivity and applicability of this system were detected.The results showed that only CTLV could be detected,while Citrus tristeza virus(CTV),Satsuma dwarf virus(SDV)and Citrus psorosis virus(CPV) can not be detected.The sensitivity of real-time PCR is higher than that of conventional PCR by 100 fold,and it can detect CTLV isolates from different kinds of citrus cultivars.Real-time RT-PCR doesn't need post-PCR handling of the amplified products such as electrophoresis,staining and imaging by completing the whole process within a single tube,and SYBR Green I dye needs low cost,so it is suitable to detect CTLV.