Expression and significance of osteopontin and integrin β_3 in human gliomas

Objective To explore the expression of osteopontin and integrin β3 in human gliomas and the correlation between osteopontin and integrinβ3.Methods The expressions of osteopontin and integrin β3 in human normal brain cells and glioma cells were detected by immunohistochemical staining,semi-quantitative reverse transcription-polymerase chain reaction(RT-PCR) and Western Blotting.Results Osteopontin and integrin β3,which were not expressed in human normal brain cells,presented in the cell membrane,cytoplasm and vascular endothelial cells of glioma.Expressions of osteopontin and integrin β3 in human brain glioma increased with increase of pathological grades of glioma(P 0.05).There was a significant positive correlation between the expressions of mRNA(r=0.990,P 0.05) and protein(r=0.943,P 0.05) of osteopontin and integrin β3.Conclusion The expressions of osteopontin and integrinβ3 are closely related to the malignant degree of glioma,furthermore,there are intrinsic association between the expressions of osteopontin and integrin β3.