Method for purifying pseudomonas aeruginosa vaccine recombinant protein Vac 9

The invention discloses a method for purifying pseudomonas aeruginosa (PA) recombinant subunit genetic engineering protein vaccine Vac 9. The Vac 9 protein is obtained in the mode that pseudomonas aeruginosa cell outer membrane protein is recombined and confused, and escherichia coli genetically engineered bacterium expression is conducted. Technologies including high pressure bacterium breaking, GST affinity chromatography, Prescission Protease (PP enzyme) restriction enzyme digestion, Phenyl HP chromatography, G25 chromatography, Q HP endotoxin removal and the like are conducted on genetic engineering bacteria, and the high-purity pseudomonas aeruginosa (PA) recombinant subunit genetic engineering protein vaccine Vac 9 is obtained. The method is simple and fast in purifying process, easy to amplify and good in repeatability, the obtained target protein is high in purity, the production requirements of genetic engineering vaccines can be met, and it is proved by animal tests that a mechanism can be effectively stimulated to generate high humoral immune response and a good immune protective effect.