Tissue Distribution of TRIM5α in Rhesus Monkey and Upregulation inPeripheral Blood Mononuclear Cell by Using Different Stimuli
2010
TRIM5α (tripartite motif protein 5-alpha) protein is a very
important restriction factor in rhesus monkey. It can restrict the
replication of retroviruses, such as HIV-1 (human immunodeficiency
virus type 1), EIAV (equine infectious anemia virus) and FIV (feline
immunodeficiency virus). Up to now, there is no report about the tissue
distribution of TRIM5α in rhesus monkey. The influence of
different stimuli on the expression of TRIM5α mRNA has also not
been examined in monkey peripheral blood mononuclear cell (PBMC). In
this study, total RNA was extracted from different tissues of a rhesus
monkey collected in Yunnan, China, and was quantified using the
β-actin gene as the internal reference. The expression levels of
TRIM5α mRNA in PBMC that was treated with three conditions (the
HIV-GFP-VSVG pseudotyped virus challenged, PMA and Ion-costimulated,
anti-CD28 and anti-CD49d antibody-costimulated) were also evaluated at
different time points. Our results showed that TRIM5α mRNA
expressed broadly in all 21 studied tissues, with the highest level in
tissues of immune system and urogenital system and a lower level in
nervous system, such as cerebrum and spinal cord. The expression level
of the TRIM5α mRNA level in PBMC was upregulated after being
activated by the three treatments.
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