Transmisson disequilibrium test for congenital dislocation of hip and Hox C gene

Objective: To investigate the correlation between the congenital dislocation of the hip (CDH) and Hox genes. Methods: A microsatellite DNA marker D12S1686 was chosen in the region of chromosome 12q13.3 where Hox C genes reside which regulate the embryonic development. Genotypes of all members in 101 CDH families were detected by the techniques of polymerase chain reaction (PCR) and denaturing polyarylamide gel electrophoresis. The transmission disequilibrium test (TDT) was performed to analyze the data of genotypes. Results: There existed 16 alleles at this locus. No transmission disequilibrium was found between CDH and alleles of D12S1686 (χ2=6.171, P =0.965). Conclusions: Hox C genes are not apparently associated with CDH.