111-P: IMPACT OF SOME NEONATAL FACTORS ON THE QUALITY OF CORD BLOOD UNITS (CBU) IN THE MEXICAN ALTRUISTIC CORD BLOOD BANK-BACECU

Aim A large number of TNC/CD34+ cells and HLA compatibility, have a selection priority for CB transplantation. Several studies have demonstrated that different obstetric and neonatal factors impact the quality of the CB units cryopreserved for BMT. The aim was to improve the CB banking process in the BACECU , analyzing parameters that may impact the concentration of TNC and/or CD34+ cells in the CBU, namely gender, weight of the infant at birth and ABO groups, since the CBU stored have unique genetic features, different from those found in other worldwide CB banks. Methods Of the 349 CBU collected at BACECU until April, 2012, 52 (14.9%) were discarded, for not complying with the minimum criteria for banking. Six were transplanted, three are reserved and 7 kept for directed medical reasons. We analyzed 281 CBU donated by Mexican mothers, who provided an informed consent and an extensive clinical summary validated by the physician. The analysis was done using the SPSS17 software and comparisons were performed with the Mann-Whitney U test. Results The frequency of ABO was: A = 35.6 %; B = 13.9 %; AB = 2.1%; O = 46.6%; We classified the following weight ranges: 4.0 kg N = 3(1.1%). Gender was evenly distributed (M = 49.8%; F = 49.8%). TNCx10 7 X = 71.29; CD34+cells (X = 2.25 × 10 6 ). X weight was 3.08 kg. No differences were found in the amount of CD34+ or TNC regarding ABO; females have an increased number of TNC (p = 0.046). CBU of babies with a weight between 3 and 4 kg have more TNC (p = 0.008, 0.028) and a NS increase of CD34s. Conclusions Although BACECU has a limited number of CBU, weight and gender impact the quality of the cells. In contrast with the data of the Korean CB bank, ABO does not have any influence. Ethnicity, weight and gender are associated with TNC yield, data in agreement with other authors and with the fact that BACECU has more Mediterranean/Caucasian than Amerindian or African genes in contrast to our BM Registry.