Self-Immolative Bioluminogenic Quinone Luciferins for NAD(P)H Assays and Reducing Capacity-Based Cell Viability Assays

Highly sensitive self-cleavable trimethyl lock quinone-luciferin substrates for diaphorase were designed and synthesized to measure NAD(P)H in biological samples and monitor viable cells via NAD(P)H-dependent cellular oxidoreductase enzymes and their NAD(P)H cofactors.