Liquid phase chip used for detecting bone metabolism biochemical marker and its preparing method

The invention discloses a liquid phase chip kit which is used for the parallel detection of a plurality of bone metabolism biochemical markers, and the invention mainly includes: coated microspheres, detection antibodies which are respectively labelled by biotin and streptavidin phycoerythrin. The liquid phase chip kit which is used for the parallel detection of a plurality of bone metabolism biochemical markers and is provided by the invention has the advantages of high detection efficiency, fewer required samples, strong specificity, high sensitivity, and so on. At the same time, all the bone metabolism biochemical markers can be combined freely, and the usage is convenient. At the same time, as all the reactions are positioned in the liquid phase environment, the invention is more conductive to the maintenance of the native conformation of protein, the reaction of a probe and the object to be detected is faster and more complete, while the detection sensitivity and the linear rangeare both greatly improved.