Extraction of sex hormone from antler velvet with supercritical CO2

Objective: To study the method of extraction of sex hormone from antler velvet with supercritical CO2.Method: Supercritical fluid extraction(SFE) was used to extract sex hormone from antler velvet and radioimmunoassay(RIA) was used to analyze the extracts.The chemical compositions in extracts were identified by GC-MS,TLC and HPLC,respectively.Result: The experimental results indicated that the extraction yield was 1.56% when 85% ethanol was used as co-solvent at temperature of 65 ℃ and extraction pressure of 30 MPa.Estradiol and progesterone in the extracts were 3.07,776.18 ng·g-1 respectively.Conclusion: It is feasible to extract hormone from antler velvet with supercritical CO2.