Влияние пробиотических штаммов эшерихий и энтерококков на активность кишечных пищеварительных ферментов при коррекции экспериментального дисбиоза у крыс

In experiments on Wistar rats, the effects of probiotic bacteria Enterococcus faecium L3 and Escerichia coli M17 on the restoration of the microbiota and activities of the intestinal digestive enzymes after experimental dysbiosis were compared. Dysbiosis was caused by intragastric administration of ampicillin and metronidazole for 3 days. Subsequently, probiotics containing E. faecium L3 (E. f.) and E. coli M17 (E. c.), or milk (control-1, K1) were administered to the animals intragastrically for 5 days. In the control K0, rats were given water, instead of antimicrobials, and then milk. Activities of the enzymes in the small intestinal mucosa were evaluated by biochemical methods, and the microbiota composition - bacteriologically and by PCR in real time. After using the E. f. there was a faster recovery of the intestinal microbiota than after using the E. c. or without probiotics (in the group K1). In the group K1, the activity of maltase, lactase, alkaline phosphatase and aminopeptidase N in the small intestine was increased in comparison with the control K0 (without antimicrobial agents and probiotics). Use of the E. f. and E. c. did not affect a recovery of the activity of aminopeptidase N, but was accompanied by a partial restoration of alkaline phosphatase activity and a tendency to restore maltase activity, and in the case of the E. f. a complete recovery of lactase activity was also observed. Thus, the probiotic E. faecium L3 bacteria, compared to the E. coli M17, are more effective in restoring the microbiota and the activity of some key intestinal enzymes (in particular lactase) after the disorders caused by intestinal dysbiosis.