FAMLF is a target of miR-181b in Burkitt lymphoma

Abstract Burkitt lymphoma (BL) is a highly malignant non-Hodgkin’s lymphoma that is closely related to the abnormal expression ofgenes. Familial acute myelogenous leukemia related factor (FAMLF; GenBank accession No. EF413001.1) is a novel gene thatwas cloned by our research group, and miR-181b is located in the intron of the FAMLF gene. To verify the role of miR-181b andFAMLF in BL, RNAhybrid software was used to predict target site of miR-181b on FAMLF and real-time quantitative PCR (RQ-PCR) was used to detect expression of miR-181b and FAMLF in BL patients, Raji cells and unaffected individuals. miR-181bwas then transfected into Raji and CA46 cell lines and FAMLF expression was examined by RQ-PCR and western blotting.Further, Raji cells viability and proliferation were detected by MTTand clone formation, and Raji cell cycle and apoptosis weredetected by flow cytometry. The results showed that miR-181b can bind to bases 21–42 of the FAMLF 5 0 untranslated region(UTR), FAMLF was highly expressed and miR-181b was lowly expressed in BL patients compared with unaffected individuals.FAMLF expression was significantly and inversely correlated to miR-181b expression, and miR-181b negatively regulatedFAMLF at posttranscriptional and translational levels. A dual-luciferase reporter gene assay identified that the 5