ITS Sequence Analysis of Nuclear Ribosomal DNA of Prunus mume in Fujian Province

To study the ITS sequence variation of Prunus mume in Fujian province from different population,the rDNA ITS regions of P.mume varieties were amplified and cloned and sequenced,and sequence data was analyzed by DNAMAN and MEGA3.1.The complete sequence of ITS and 5.8S rDNA of 18 different P.mume population were obtained.The sequences of ITS1,5.8S and ITS2 are 223～224 bp,164 bp and 241～246 bp,respectively,5.8S is more conservative than ITS.Phylogenetic tree based on ITS sequences data was conducted by UPGMA method,which shows the variation at the level of molecular biology.It provided the basis for variety identification of P.mume.And 14 rDNA ITS sequences of P.mume were deposited in GenBank under number EF523482-EF523493,EF529435 and EF529436.