Callus Induction and Shoot Regeneration of Strawberry 'Tochiotome'

Optimal conditions for callus induction and shoot regeneration in the strawberry cultivar ‘Tochiotome’ were studied using in vitro culture of plant leaves. Callus induction on a 1/3-strength MS medium supplemented with sucrose (30g/L), TDZ (1.0mg/L), and 2, 4D (0.1mg/L) was found to be the most optimal condition, whereas a full-strength MS medium supplemented with sucrose (30g/L) and TDZ (1.0mg/L) was found to be the most optimal condition for shoot regeneration. The youngest two leaves of plants transplanted from in vitro cultures for ~1 month to hormone-free MS medium were suitable for use as explants. This leaf culture method can supply regenerated plants throughout the year. As for the anther culture method, regenerated plants were obtained by culturing anthers from flower buds containing unicellular pollen on LS medium supplemented with sucrose (3g/L), BA (2.0mg/L), and NAA (0.02mg/L).