EXPERIMENTAL STUDY ON EFFECTS JIECHANG KANGTAI ON APOPTOSIS AND EXPRESSION OF CORRELATIVE REGULATIVE GENES IN COLONIC MUCOUS CELLS OF COLITIS RATS

Objective:To discuss the effects of jiechang kangtai(JCKT) on apoptosis and expression of Bcl-2,c-Myc and P~(53) gene protein in colonic mucous cells of colitis rats. Methods:60 female SD rats were randomly divided into four groups:normal,model,solfasalazine(SASP) and JCKT groups.Experimental colitis models were induced by trinitrobenzene sulphonic acid(TNBs).The apoptosis of cells were tested by TUNEL and the expression of Bcl -2,c-Myc and p~(53) gene protein were observed by immunohistochemistry. Results:The AI,expression of c-Myc and P~(53) in the two treated groups(JCKT,SASP) were obviously lower than those in the model group, the expression of Bcl-2 in the two treated groups were higher than that in the model group(P0.05). Conclusion:JCKT can inhibit the apoptosis of colonic mucous cells in colitis rats which maybe by means of regulating the expression of Bcl -2,c-Myc and P~(53) genes protein.