Cloning and Sequence Analysis of EoblGOBP in the Antennal of Ectropis obliqua hypulina

The full-length sequences of two general odorant binding protein(GOBP) genes in Ectropis obliqua(named EoblGOBP1 and EoblGOBP2) were cloned with degenerated digonudeotide primers by RT-PCR and the rapid amplification of cDNA ends(RACE) using the total RNA isolated from the antenna of Ectropis obliqua as template.The length of EoblGOBP1 was 1 528 bp with a 501bp ORF which encoded a peptide of 166 amino acid residues.The predicted Mw and pI of the peptide were 18 835.63 D and 5.45 respectively.The NCBI/GenBank accession numbers of cDNA and amino acid of EoblGOBP1were FJ156732 and ACN29680.1,respectively.The length of EoblGOBP2 was 1 315 bp with a 405 bp ORF which encoded a peptide of 160 amino acid residues.The predicted Mw and pI of the peptide were 18 002.75 D and 5.32 respectively.The GenBank accession numbers of cDNA and amino acid of EoblGOBP2 were FJ 156733 and ACN29681.1,respectively.The amino acid sequences from the two gene sequences had 6 typical conservative Cys.Blast searching in NCBI showed that the amino acid sequences were highly similar to that of other GOBPs from Lepidoptera with sequence identities of 62%～82% and 76%～88%.