Ameliorating effects of essential oil from Fructus Apliniae zerumbet(EOFAZ) on NOS-NO system in human umbilical vein endothelial cells injury(HUVECs) induced by oxidized-low density lipoprotein(ox-LDL) in vitro

OBJECTIVE To investigate the protective effects of essential oil from Fructus Apliniae zerumbet(EOFAZ) on human umbilical vein endothelial cells(HUVECs) injury induced by oxidized low density lipoprotein(ox-LDL),and explore the ameliorating effects on NOS-NO system.METHODS The in vitro cultured HUVECs were pre-incubated with 0.1 and 0.01 μg·mL-1 EOFAZ 24 h,and then exposure to ox-LDL 24 h.The cyto-morphological change was observed by inverted phase contrast microscope and Giemsa staining assay.The NO content in medium and NOS subtype activities in cell lysis were assayed by colorimetric methods according to the instruction of commercial kits.RESULTS After exposuring to ox-LDL for 24 h,the numbers and morphology of HUVECs were significantly changed by the inverted phase contrast microscope and Giemsa staining compared with control group.The NO contents in medium were significantly decrease compared with control group,the tNOS and eNOS activities of cellular lysis as well.Pre-treated with EOFAZ which ameliorated the cytomorphology and increased the number of HUVECs,significantly enhanced the activities of tNOS and eNOS of cell lysis,and the NO contents in medium.However,there were no any significant changes of iNOS activity whether in model group or pre-treated with EOFAZ.CONCLUSION EOFAZ can ameliorate the HUVECs injury induced by ox-LDL,and the mechanism maybe related to enhancement of eNOS activity of cells and promotion of promote NO secretion.