Validation of potato cyst nematode resistant genotypes through molecular markers

Forty-two potato genotypes exhibiting phenotypic resistance against Potato Cyst Nematode (PCN) species Globodera rostochiensis (golden) and Globodera pallida (white) were screened for molecular validation using tightly linked genetic DNA markers. H1 and GroV1 gene conferring resistance against golden nematodes were validated by TG 689 & 57R and X02 markers, respectively. Similarly, resistance against Globodera pallida governed by QTL GpaVvrn and QTLs Gpa5, Gpa6, was confirmed by corresponding markers HC and SPUD1636. On the basis of phenotypic and genotypic confirmation, this study identifies CP 1843, 1879 and JEX/A 267 as elite potato genotypes that can be utilized as parental lines for introgression of resistant genes against both PCN species.