Plant Regeneration in Tissue and Protoplast Cultures of Ipomoea cordatotriloba

To utilize stem nematode resistance gene of Ipomoea cordatotriloba, a wild relative of sweetpotato (Ipomoea batatas (L.) Lam.), through somatic hybridization, an efficient system of plant regeneration from protoplasts of this relative has been established in the present study. Petiole tissues of I. cordatotriloba formed calluses on MS medium with 0.05 mg/L 2,4-D and 0.5 mg/L KT. The obtained calluses were cultured on the six regeneration media and the highest regeneration frequency up to 52.00% was achieved on the regeneration medium with 0.1 mg/L IAA and 5.0 mg/L BAP. Petiole protoplasts of I. cordatotriloba gave a regeneration frequency up to 50.98% through the use of petiole culture system of this relative.