Validation of the VERSA 10 Workstation for Real-Time PCR Setup using cDNA from two Genes of Trout Fish

template DNA thereby decreasing the risk of cross-contamination, while achieving accuracy and reproducibility. Aurora Biomed Inc. has recently launched the compact, multi-channel VERSA 10 PCR Setup Workstation. Samples, Master Mixes and Primers T IP W aste (1 μL of cDNA) provide confidence of variability (CV%) values ranging from 0.26 to 1.79, indicating very precise liquid handling. Similarly, the CV% values among the 8 channels ranged from 0.4 to 1.62 suggesting a high The validation data on this system is herein presented. Figure 2: Deck layout of the VERSA 10 PCR Setup Workstation degree of accuracy for all 8 channels of the workstation. Furthermore, low CV% values were obtained in the amplification of the SF1a gene where the replication and inter-channel CV% range was 1.15 to 3.17 and 0.45 to 2.69, Figure 4: Amplification plots and dissociation curves from the