Genetic differences between Chabertia ovina and C. erschowi revealed by sequence analysis of four mitochondrial genes.

AbstractThis study examined sequence differences in mitochondrial cytochrome c oxidase subunit 1 (cox1), large subunit ribosomal RNA (rrnL) and NADH dehydrogenase subunits 1 and 4 (nad1 and nad4) between Chabertia ovina and C. erschowi from yaks in Qinghai and goats in Shaanxi provinces, China. A part of the cox1 (pcox1), rrnL (prrnL), nad1 and nad4 genes (pnad1 and pnad4) were amplified separately from individual nematodes by PCR and sequenced. The length of the sequences of pcox1, prrnL, pnad1 and pnad4 was 441 bp, 450 bp, 526 bp and 914 bp for C. ovina, and 441 bp, 451 bp, 517 bp and 810 bp for C. erschowi, respectively. The intra-specific sequence variations within C. ovina were 0.2–2.9% for pcox1, 0–0.9% for prrnL, 0.6–2.3% for pnad1, and 0.4–2.0% for pnad4, and were 0.5–1.6% for pcox1, 0–1.1% for prrnL, 0.2–1.7% for pnad1, and 0.4–1.1% for pnad4 within C. erschowi. Whereas, the inter-specific sequence differences between the two species were obviously higher, being 11.6–12.9% for pcox1, 9.8–11.1% fo...