Rapid extraction method for detection of outer membrane protein F of Pseudomonas aeruginosa

Abstract A filtration technique has been developed to trap the peptidoglycan sacculus for rapid extraction of the outer membrane protein F of Pseudomonas aeruginosa . The method consists of the following three steps: ( i ) removal of cell components except peptidoglycan associated with some proteins, ( ii ) trapping of peptidoglycan with a glass filter and ( iii ) extraction with a hot SDS solution of proteins, mainly F protein, associated with peptidoglycan. The method is simple and rapid, providing for efficient screening for protein F-deficient mutants of P. aeruginosa . Using this method of screening, three mutants were isolated among 500 mutagenized clones.