The UniformMu Resource: Construction, Applications, and Opportunities

Invaluable insights into functional genomics have arisen from knockout and knockdown mutants generated by transposon mutagenesis. Thousands of insertional mutants are available free of charge from the UniformMu national public resource for maize. This resource was created using the native Robertson’s Mutator system and resulting features include (1) an inbred genetic background ideal for phenotype analysis; (2) effective genetic control of Mu transposon activity that facilitates genetic and molecular analyses; (3) precise mapping of transposon insertions enabled by targeted sequencing (Mu flanks identified by a Mu-seq protocol for high-throughput genotyping); (4) cost-free, worldwide distribution of high-quality, sustainable seed stocks through MaizeGDB.org and the Maize Genetics Cooperation Stock Center. Available materials have been especially useful for genetic analysis of complex, multi-genetic traits such as domestication, seed development, and disease resistance. Additional applications for UniformMu resources include new strategies for both forward- and reverse-genetics (phenotype-to-genotype or the reverse) as well as synergies with emerging gene-editing technologies (e.g., MuCRISPR).