Sample gradient illuminating method and device for light-transmitting optical microscope

The invention discloses a sample gradient illuminating method for a light-transmitting optical microscope and a sample gradient illuminating device realizing the method for the light-transmitting optical microscope. The sample gradient illuminating device comprises a first convex lens, a second convex lens, a spatial filter and a heat radiating fin. According to the sample gradient illuminating method and the sample gradient illuminating device disclosed by the invention, the contrast ratio of transmitting light penetrating through a sample during imaging is remarkably enhanced by using the generated gradient light intensity illumination microscope through the filtering action of the spatial filter and the smooth action of the heat radiating fin. According to the sample gradient illuminating method and the sample gradient illuminating device disclosed by the invention, experimenters can clearly recognize cells and accurately position the trends of cell bodies, dendrite and axone.