Automated Profiling of Multiplexed DNA Markers. An Italian Database of Four Coamplified STR Loci

Automated DNA profiling by fluorescent-based technology and its application on multiplex amplified polymerase chain reaction (PCR) products have been studied and validated for use in forensic laboratory routine [1–5]. The most diffused protocol provides coamplification of 4 short tandem repeat (STR) loci labelled by 2 different fluorescent dye markers and separation of PCR products by electrophoresis on an automated DNA sequencer 373A Leon (Applied Biosystems).