An Improved Method of Extracting Total RNA from Animal Tissues Adapted to Experimental Teaching

To explore the method of extracting total RNA from animal tissues adapted to experimental teaching,an improved method which used chloroform to treat the utensils,consumables and water to decrease the degradation of exogenous RNA enzymes was established in this study.The results showed that the isolated RNA was intact and suitable for further molecular applications by RT-PCR.This indicates that the method was appropriate for RNA extraction of animal tissues and adapted to experimental teaching.